Composite

Part:BBa_M36719:Design

Designed by: Tammy Hsu, Ruby Lee   Group: Stanford BIOE44 - S11   (2011-04-28)


medium-chain alkane hydroxylating device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2235
    Illegal PstI site found at 2617
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2235
    Illegal PstI site found at 2617
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 444
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2235
    Illegal PstI site found at 2617
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2235
    Illegal PstI site found at 2617
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1216
    Illegal NgoMIV site found at 2025
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2303
    Illegal BsaI site found at 2445


Design Notes

N/A


Source

alkB2, rubA1, rubA2 are from Pseudomonas aeruginosa PAO1. rubB is from Pseudomonas fluorescens Pf-5.

References